Protein_Domain

Part:BBa_K1733001:Design

Designed by: Jeffrey Chen   Group: iGEM15_BostonU   (2015-09-17)

ABI Dimerizable Domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Originally, this protein was not biobrick compatible, containing one of the restriction sites in the sequence. We therefore had to introduce a silent mutation in the sequence, changing the nucleotides but keeping the amino acid sequence, and we ordered the new synthesized part from IDT. We then constructed the biobrick part using a PCR reaction and ligation into the psb1c3 backbone.


Source

Arabidopsis thaliana

References